CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB, IF, FC (Intra)

Host / Isotype

Mouse / IgG2b


human, mouse, rat, hamster, zebrafish, monkey, canine


WB, IF, FC (Intra)


CoraLite® Plus 488 Fluorescent Dye



Cat no : CL488-66009


ACTB, actin, actin, beta, Beta Actin

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells
Positive IF detected inMDCK cells, U2OS cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66009 targets Beta Actin in WB, IF, FC (Intra) applications and shows reactivity with human, mouse, rat, hamster, zebrafish, monkey, canine samples.

Tested Reactivity human, mouse, rat, hamster, zebrafish, monkey, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name actin, beta
Calculated Molecular Weight 42 kDa
GenBank Accession NumberNM_001101
Gene Symbol ACTB
Gene ID (NCBI) 60
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. And the anitbody is CL488 (Ex/Em 488 nm/515 nm) conjugated.


Product Specific Protocols
WB protocol for CL Plus 488 Beta Actin antibody CL488-66009Download protocol
IF protocol for CL Plus 488 Beta Actin antibody CL488-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols



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