CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for FC (Intra), IF, WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster, zebrafish, monkey

Applications

WB, IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D4H5

Cat no : CL488-66009

Synonyms

ACTB, actin, actin, beta, Beta Actin



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells
Positive IF detected inMDCK cells, U2OS cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66009 targets Beta Actin in WB, IF, FC (Intra) applications and shows reactivity with human, mouse, rat, hamster, zebrafish, monkey samples.

Tested Reactivity human, mouse, rat, hamster, zebrafish, monkey
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name actin, beta
Calculated molecular weight 42 kDa
GenBank accession numberNM_001101
Gene symbol ACTB
Gene ID (NCBI) 60
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. And the anitbody is CL488 (Ex/Em 488 nm/515 nm) conjugated.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 Beta Actin antibody CL488-66009Download protocol
IF protocol for CL Plus 488 Beta Actin antibody CL488-66009Download protocol
FC protocol for CL Plus 488 Beta Actin antibody CL488-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Br J Pharmacol

Fingolimod ameliorates schizophrenia-like cognitive impairments induced by phencyclidine in male rats

Authors - Xueli Yu
humanWB

Mediators Inflamm

Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2

Authors - Junqi Fu
mouseIF

Nanoscale

Effects of hydrophilic fullerene nanoarchitectured structures on the behaviour of neural stem cells.

Authors - Chui-Wei Wong