Product Information
66009-1-PBS targets Beta Actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, canine, monkey, chicken, zebrafish, hamster samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit, canine, monkey, chicken, zebrafish, hamster |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | actin, beta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001101 |
| Gene Symbol | Beta Actin |
| Gene ID (NCBI) | 60 |
| RRID | AB_2687938 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P60709 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used "housekeeping gene" in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.
What is the molecular weight of beta-actin?
42 kDa
What is the function of beta-actin?
Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).
What are the applications for beta-actin?
As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.
Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).
What are the post-transcriptional modifications (PTMs) to beta actin?
Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer's disease (PMID: 11246152).
For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.
Publications
What published studies show
The Beta Actin Monoclonal antibody (catalog number 66009-1-PBS, PBS Only) has a total of 12,943 citations. These appear across high-impact journals including Nature, Cell, Cancer Cell, Science, Signal Transduction and Targeted Therapy, Cell Metabolism, Immunity, Nature Metabolism, Nature Cancer, Nature Immunology, Molecular Cancer, Gastroenterology, and Advanced Materials. The associated research fields span cancer biology, metabolism, immunology, neurodegeneration, inflammation, and regenerative medicine, with Western blot as the predominant application.
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces Biomimetic Targeted Nanoplatform Exerts a Combined Effect of Alleviating Calcium Overload and Scavenging ROS to Attenuate the Inflammatory Response in Myocardial Ischemia-Reperfusion Injury | ||
J Virol Porcine reproductive and respiratory syndrome virus nsp5 inhibits the activation of the Nrf2/HO-1 pathway by targeting p62 to antagonize its antiviral activity | ||
J Mol Endocrinol Protective effect of pentoxifylline against high-glucose-induced ferroptosis in vascular smooth muscle cells. | ||
Exp Cell Res Hydrostatic compressive stress promotes MC3T3-E1 cell proliferation through ROCK-dependent regulation of Cyclin D1 and CDK4. |
