CoraLite®594-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for FC (Intra), IF, WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster, zebrafish, monkey, canine

Applications

WB, IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D4H5

Cat no : CL594-66009

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells
Positive IF detected inMDCK cells, HepG2 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 14 publications below

Product Information

CL594-66009 targets Beta Actin in WB, IF, FC (Intra) applications and shows reactivity with human, mouse, rat, hamster, zebrafish, monkey, canine samples.

Tested Reactivity human, mouse, rat, hamster, zebrafish, monkey, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name actin, beta
Calculated Molecular Weight 42 kDa
GenBank Accession NumberNM_001101
Gene Symbol ACTB
Gene ID (NCBI) 60
RRIDAB_2883475
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b. And the anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Protocols

Product Specific Protocols
WB protocol for CL594 Beta Actin antibody CL594-66009Download protocol
IF protocol for CL594 Beta Actin antibody CL594-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Protein Cell

The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting

Authors - Yuhan Chen
mouseWB

J Clin Invest

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Authors - Zhixiong Liu
WB

Hypertens Res

Unilateral chemical ablation of the adrenal gland lowers blood pressure and alleviates target organ damage in spontaneously hypertensive rats

Authors - Xinquan Wang
WB

Inflammation

Ursolic Acid Promotes Autophagy by Inhibiting Akt/mTOR and TNF-α/TNFR1 Signaling Pathways to Alleviate Pyroptosis and Necroptosis in Mycobacterium tuberculosis-Infected Macrophages

Authors - Jingjing Shen
WB

Neurochem Res

Akt/mTOR Pathway Agonist SC79 Inhibits Autophagy and Apoptosis of Oligodendrocyte Precursor Cells Associated with Neonatal White Matter Dysplasia

Authors - Zhongni Li
mouseWB

J Ethnopharmacol

The licorice flavonoid isoliquiritigenin attenuates Mycobacterium tuberculosis-induced inflammation through Notch1/NF-κB and MAPK signaling pathways.

Authors - Jinxia Sun