Pan-Keratin (TypeⅡ) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86370-1-RR (same clone as 86370-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 86370-1-RR (same clone as 86370-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 86370-1-RR (same clone as 86370-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 86370-1-RR (same clone as 86370-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 86370-1-RR (same clone as 86370-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

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  IF Staining of human cervical cancer using 86370-1-RR (same clone as 86370-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 86370-1-RR (same clone as 86370-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 86370-1-RR (same clone as 86370-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 86370-1-RR (same clone as 86370-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 86370-1-RR (same clone as 86370-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

Pan-Keratin (TypeⅡ) Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 86370-1-PBS

Clone No.251040F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Pan Keratin, Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins

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Product Information

86370-1-PBS targets Pan-Keratin (TypeⅡ) in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Pan-Keratin (TypeⅡ)
• Observed Molecular Weight: 52-68 kDa
• Gene ID (NCBI): Pan-Keratin (TypeⅡ)
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14533
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type II keratins (or Type II cytokeratins) are basic or neutral proteins that form part of the intermediate filament cytoskeleton in epithelial cells. Type II keratins include K1-K8, and K71-K86.