Pan-Keratin (Type I) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86238-2-RR (same clone as 86238-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human cervical cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

Pan-Keratin (Type I) Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 86238-2-PBS

Clone No.251039C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Pan Keratin, pan-CK, Pan-CK (Type I), Pan-Keratin, Type I keratin

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Product Information

86238-2-PBS targets Pan-Keratin (Type I) in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Pan-Keratin (Type I)
• Observed Molecular Weight: 45-65 kDa
• Gene ID (NCBI): Pan-Keratin (Type I)
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15323
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40.