Tested Applications
| Positive WB detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31736-1-AP targets Pannexin 3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34451 Product name: Recombinant human PANX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 289-392 aa of NM_052959 Sequence: YNLTRLCRWDKRLLSVYEMLPAFDLLSRKMLGCPINDLNVILLFLRANISELISFSWLSVLCVLKDTTTQKHNIDTVVDFMTLLAGLEPSKPKHLTNSACDEHP Predict reactive species |
| Full Name | pannexin 3 |
| Calculated Molecular Weight | 392 aa, 45 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | NM_052959 |
| Gene Symbol | PANX3 |
| Gene ID (NCBI) | 116337 |
| RRID | AB_3742466 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96QZ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pannexin 3 (PANX3) is a member of the pannexin family of single membrane channel-forming glycoproteins. PANX3 is expressed in osteoblasts, chondrocytes, and skin and plays a major role in calcium homeostasis suggesting an independent functional niche. PANX3 regulates both chondrocyte and osteoblast differentiation via the activation of intracellular Ca2+ signaling pathways through multiple channel activities: hemichannels, endoplasmic reticulum (ER) Ca2+ channels, and gap junctions. PANX3 has 392 amino acids with a molecular weight of ~ 43 kDa and is N-linked glycosylated at asparagine residue 71 in the first extracellular loop (PMID: 27837015, PMID: 34250568, PMID: 38580658).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Pannexin 3 antibody 31736-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
