Pannexin 3 Polyclonal antibody

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  WB analysis using 31736-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 31736-1-AP (Pannexin 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Pannexin 3 Polyclonal Antibody for WB, ELISA

Cat No. 31736-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PANX3, PX3

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Tested Applications

• Positive WB detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31736-1-AP targets Pannexin 3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Pannexin 3 fusion protein Ag34451
• Full Name: pannexin 3
• Calculated Molecular Weight: 392 aa, 45 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: NM_052959
• Gene Symbol: PANX3
• Gene ID (NCBI): 116337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96QZ0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pannexin 3 (PANX3) is a member of the pannexin family of single membrane channel-forming glycoproteins. PANX3 is expressed in osteoblasts, chondrocytes, and skin and plays a major role in calcium homeostasis suggesting an independent functional niche. PANX3 regulates both chondrocyte and osteoblast differentiation via the activation of intracellular Ca2+ signaling pathways through multiple channel activities: hemichannels, endoplasmic reticulum (ER) Ca2+ channels, and gap junctions. PANX3 has 392 amino acids with a molecular weight of ~ 43 kDa and is N-linked glycosylated at asparagine residue 71 in the first extracellular loop (PMID: 27837015, PMID: 34250568, PMID: 38580658).