Paxillin Polyclonal antibody

• 

  WB analysis using 10029-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 32083-1-AP (Paxillin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Paxillin Polyclonal Antibody for WB, ELISA

Cat No. 32083-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PXN

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Tested Applications

• Positive WB detected in: A431 cells, COLO 320 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32083-1-AP targets Paxillin in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33616

  Product name: Recombinant human Paxillin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of NM_001080855

  Sequence: MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGG*

• Full Name: paxillin
• Calculated Molecular Weight: 68kd
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_001080855
• Gene Symbol: Paxillin
• Gene ID (NCBI): 5829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49023
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).