Pericentrin Polyclonal antibody, PBS Only

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• Pericentrin Polyclonal antibody, PBS Only (22271-1-PBS)

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  IHC staining of human intrahepatic cholangiocarcinoma using 22271-1-AP (same clone as 22271-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22271-1-AP (Pericentrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 22271-1-AP (same clone as 22271-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 22271-1-AP (Pericentrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 22271-1-AP (same clone as 22271-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22271-1-AP (same clone as 22271-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22271-1-AP (same clone as 22271-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

Pericentrin Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 22271-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

22271-1-PBS targets Pericentrin in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: pericentrin
• Calculated Molecular Weight: 378 kDa
• GenBank Accession Number: NM_006031
• Gene Symbol: Pericentrin
• Gene ID (NCBI): 5116
• RRID: AB_2879056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95613
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.