Periphilin 1 Polyclonal antibody

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  WB analysis using 30437-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30437-1-AP (Periphilin 1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 30437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30437-1-AP (Periphilin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 30437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30437-1-AP (Periphilin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Periphilin 1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30437-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PPHLN1, Gastric cancer antigen Ga50, HSPC206, HSPC232, Periphilin-1

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Tested Applications

• Positive WB detected in: A549 cells, Calu-1 cells, Jurkat cells, MG-63 cells, U2OS cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30437-1-AP targets Periphilin 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32768

  Product name: Recombinant human PPHLN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 33-200 aa of BC039832

  Sequence: ESGYRWTRDDHSASRQPEYRDMRDGFRRKSFYSSHYARERSPYKRDNTFFRESPVGRKDSPHSRSGSSVSSRSYSPERSKSYSFHQSQHRKSVRPGASYKRQNEGNPERDKERPVQSLKTSRDTSPSSGSAVSSSKVLDKPSRLTEKELAEAASKWAAEKLEKSDESN

• Full Name: periphilin 1
• Calculated Molecular Weight: 458 aa, 53 kDa
• Observed Molecular Weight: 37-70 kDa
• GenBank Accession Number: BC039832
• Gene Symbol: PPHLN1
• Gene ID (NCBI): 51535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEY8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Periphilin 1 is a component of the HUSH complex, which is a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is likely necessary for maintaining transcriptional silencing by promoting the recruitment of SETDB1, a histone methyltransferase that mediates the deposition of additional H3K9me3. Periphilin 1 contributes to the maintenance of the HUSH complex at chromatin (PMID: 26022416).