Phosphatase Inhibitor Cocktail (100X)

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  NIH3T3 cells were activated with 400 nM PMA for 15 min. Cells were then lysed using RIPA Buffer (PR20135) with (lanes 1 and 2) and without (lanes 3 and 4) phosphatase inhibitor (PR20015). Cell lysates were then incubated at 37°C for 4 hours (lanes 1 and 3) or 21 hours (lanes 2 and 4). Following this incubation lysates were subjected to SDS-PAGE (5 ug protein/well) followed by western blot with anti-phospho-ERK1/2 (Thr202/Tyr204; 28733-1-AP).

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Cat No. PR20015

Publications

56

Concentration

100x

Component

sodium fluoride，sodium orthovanadate，Sodium Pyrophosphate，β-glycerophosphate，Imidazole，Sodium molybdate，Sodium tartrate dibasic dihydrate

Subject

Primary cells, mammalian cultured cells, animal tissues, plant tissues, yeast or bacteria

Usage

Protein purification, western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry and other studies

Specificity

Highly potent, broad-spectrum

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Product Information

Proteintech's Phosphatase Inhibitor Cocktail is a highly potent, broad-spectrum cocktail containing multiple phosphatase inhibitors. The main components include sodium fluoride, sodium orthovanadate, sodium pyrophosphate, beta-glycerophosphate, imidazole, sodium molybdate, sodium tartrate dibasic dihydrate, and other inhibitors, mainly inhibiting serine/threonine phosphatases, acid phosphatases, alkaline phosphatases, tyrosine phosphotases, etc. The cocktail can effectively prevent protein dephosphorylation during the extraction or lysis of primary cells, mammalian cultured cells, animal tissues, plant tissues, yeast or bacteria. It can be used for multiple applications including protein purification, western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry and other studies.

Components

The main components include sodium fluoride, sodium orthovanadate, sodium pyrophosphate, beta-glycerophosphate, imidazole, sodium molybdate, sodium tartrate dibasic dihydrate, and other inhibitors, mainly inhibiting serine/threonine phosphatases, acid phosphatases, alkaline phosphatases, tyrosine phosphotases, etc.

Storage

Store at -20°C for up to one year.

Usage

Dissolve well at room temperature and add to the lysate or sample solution at a dilution ratio of 1:100 or according to the needs of the experiment.

Notes

1. The phosphatase content can differ across samples. Therefore, the amount of phosphatase inhibitor cocktail can be adjusted according to the experimental situation.

2. This product is a highly concentrated saturated solution. Due to temperature fluctuations, a small amount of crystallisation may occur during storage or use. This is a normal physical phenomenon and does not affect the product's quality or normal use.

3. Dissolve at room temperature, do not heat.

4. For your safety and health, please wear lab coats and disposable gloves.

Cited in Article as

PR20015, Phosphatase Inhibitor Cocktail (100X), Proteintech, IL, USA

Publications

Application	Title
	Immunity; Maternal natural killer cells drive neuroimmune disorders in offspring through aberrant secretion of extracellular granzyme B; Authors - Qiwu Bian
	Gut Microbes; Probiotic Lactobacillus casei improves immune microenvironment in rheumatoid arthritis via gut microbiota-butyrate-HDAC/NF-κB signaling.; Authors - Yanmiao Ma
	J Nanobiotechnology; Poria cocos-derived exosome-like nanoparticles ameliorate lymphedema by reprogramming fibroblast metabolism via enhanced TCA cycle flux.; Authors - Haotian Liu
	Adv Sci (Weinh); Snord15b Maintains Stemness of Intestinal Stem Cells via Enhancement of Alternative Splicing of Btrc Short Isoform for Suppression of β-Catenin Ubiquitination; Authors - Yuwei Xu
	Pharmacol Res; Desaminotyrosine alleviates hepatic ischemia-reperfusion injury via the MAPK/ferroptosis axis and Bifidobacterium pseudolongum modulation.; Authors - Junyang Zhou
	Phytomedicine; Que Zui tea extract alleviates atherosclerosis via liver-vasculature-gut axis by modulating lipid metabolism, inflammation, and gut microbiota in ApoE(-/-) mice.; Authors - Xinjie Chen