Phospho-DLK/MAP3K12 (Thr43) Polyclonal antibody

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• Phospho-DLK/MAP3K12 (Thr43) Polyclonal antibody (28277-1-AP)

Phospho-DLK/MAP3K12 (Thr43) Polyclonal Antibody for WB, ELISA

Cat No. 28277-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Synonyms

DLK, Leucine zipper protein kinase, MAP3K12, MAPK upstream kinase, Mixed lineage kinase, MUK, Phospho-DLK/MAP3K12 (Thr43), ZPK, ZPKP1

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  WB analysis using 28277-1-AP

  Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28277-1-AP (Phospho-DLK (Thr43) antibody) at dilution of 1:600 incubated at 4℃ overnight.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PDGF treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28277-1-AP targets Phospho-DLK/MAP3K12 (Thr43) in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase kinase 12
• Calculated Molecular Weight: 93 kda
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: NM_001193511
• Gene Symbol: MAP3K12
• Gene ID (NCBI): 7786
• RRID: AB_2918153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12852
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K12, also named as DLK ((dual leucine zipper kinase), is a part of a non-canonical MAPK signaling pathway. It has been reported that MAP3K12 can be activated by APOE through a receptor-mediated mechanism. MAP3K12 enhances the AP-1-mediated transcription of APP via a MAP kinase signal transduction pathway composed of MAP2K7, MAPK1/ERK2 and MAPK3/ERK1. MAP3K12 is required for pancreatic beta-cell proliferation during postnatal development and stress-induced JNK signaling in neurons.(PMID: 28111074, 25341110, 32189007)

Publications

What published studies show

Phospho-DLK/MAP3K12 (Thr43) Polyclonal antibody (Cat# 28277-1-AP) has 2 citations. One appeared in European Journal of Pharmacology (2024), studying MAPK genes in renal clear cell carcinoma prognosis and drug treatment using bioinformatics and Mendelian randomization. The other was published in Investigative Ophthalmology & Visual Science (2025), examining HDAC4-mediated neuroprotection of retinal ganglion cells after optic nerve injury. The associated research fields span oncology/bioinformatics and neuroscience/ophthalmology.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Eur J Pharmacol; Comprehensive analysis of MAPK genes in the prognosis, immune characteristics, and drug treatment of renal clear cell carcinoma using bioinformatic analysis and Mendelian randomization; Authors - Xinyi Zheng ; KD Validated
mouse	IF	Invest Ophthalmol Vis Sci; HDAC4 Promotes Neuroprotection of Retinal Ganglion Cells After Optic Nerve Injury.; Authors - Ying Zhu