Phospho-DLK/MAP3K12 (Thr43) Polyclonal antibody

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• Phospho-DLK/MAP3K12 (Thr43) Polyclonal antibody (28277-1-AP)

Phospho-DLK/MAP3K12 (Thr43) Polyclonal Antibody for WB, ELISA

Cat No. 28277-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Synonyms

DLK, Leucine zipper protein kinase, MAP3K12, MAPK upstream kinase, Mixed lineage kinase, MUK, Phospho-DLK/MAP3K12 (Thr43), ZPK, ZPKP1

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  WB analysis using 28277-1-AP

  Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28277-1-AP (Phospho-DLK (Thr43) antibody) at dilution of 1:600 incubated at 4℃ overnight.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PDGF treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28277-1-AP targets Phospho-DLK/MAP3K12 (Thr43) in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase kinase 12
• Calculated Molecular Weight: 93 kda
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: NM_001193511
• Gene Symbol: MAP3K12
• Gene ID (NCBI): 7786
• RRID: AB_2918153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12852
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K12, also named as DLK ((dual leucine zipper kinase), is a part of a non-canonical MAPK signaling pathway. It has been reported that MAP3K12 can be activated by APOE through a receptor-mediated mechanism. MAP3K12 enhances the AP-1-mediated transcription of APP via a MAP kinase signal transduction pathway composed of MAP2K7, MAPK1/ERK2 and MAPK3/ERK1. MAP3K12 is required for pancreatic beta-cell proliferation during postnatal development and stress-induced JNK signaling in neurons.(PMID: 28111074, 25341110, 32189007)

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Invest Ophthalmol Vis Sci; HDAC4 Promotes Neuroprotection of Retinal Ganglion Cells After Optic Nerve Injury.; Authors - Ying Zhu
human	WB	Eur J Pharmacol; Comprehensive analysis of MAPK genes in the prognosis, immune characteristics, and drug treatment of renal clear cell carcinoma using bioinformatic analysis and Mendelian randomization; Authors - Xinyi Zheng ; KD Validated