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CoraLite®555-conjugated Phospho-AKT (Ser473) Recombinant antibody

Phospho-AKT (Ser473) Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2E17

Cat no : CL555-80455

Synonyms

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®555-conjugated Phospho-AKT (Ser473) Recom (CL555-80455)

    FC experiment of HEK-293 using CL555-80455

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®555 Anti-Human Phospho-AKT (Ser473) (CL555-80455, Clone:2E17), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®555-conjugated Phospho-AKT (Ser473) Recom (CL555-80455)

    FC experiment of HEK-293 using CL555-80455

    1X10^6 HEK-293 cells untreated (left) or treated with Calyculin A (right) were intracellularly stained with 0.13 ug CoraLite®555 Anti-Human Phospho-AKT (Ser473) (CL555-80455, Clone:2E17), or 0.13 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

Tested Applications

Positive FC detected inCalyculin A treated HEK-293 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-80455 targets Phospho-AKT (Ser473) in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name v-akt murine thymoma viral oncogene homolog 1
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_005163
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672)