Phospho-AKT (Thr450) Recombinant monoclonal antibody

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  WB analysis using 87220-1-RR

  Non-treated MCF-7 cells, phosphatase inhibitor treated MCF-7 cells and λ phosphatase treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87220-1-RR (Phospho-AKT (Thr450) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-AKT (Thr450) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87220-1-RR

Clone No.252456B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

AKT (phospho T450), AKT1 (phospho T450), p-AKT, p-AKT (Thr450), p-AKT T450

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Tested Applications

• Positive WB detected in: MCF-7 cells, λ phosphatase treated MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87220-1-RR targets Phospho-AKT (Thr450) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-akt murine thymoma viral oncogene homolog 1
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_005163
• Gene Symbol: AKT1
• Gene ID (NCBI): 207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P31749
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Phospho-AKT (Thr450) is recognized as the phosphorylation site on AKT (protein kinase B) at the threonine 450 residue. This site is referred to as the turn phosphorylation site and was previously thought to be a result of autophosphorylation, but more recent work has shown that its phosphorylation is controlled by TORC2 activity. This phosphorylation site is important in other AGC kinases, but the role in AKT signaling had not been determined. (PMID: 21670491)