Phospho-ALK (Tyr1278) Recombinant monoclonal antibody

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• Phospho-ALK (Tyr1278) Recombinant monoclonal antibody (87874-1-RR)

Phospho-ALK (Tyr1278) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87874-1-RR

Clone No.253445D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ALK (Tyr1278), p-ALK (Tyr1278), pALK Y1278, Phospho-ALK, Phospho-NPM-ALK

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  WB analysis of Karpas-299 using 87874-1-RR

  Non-treated and pervanadate treated Karpas-299 cells were subjected to SDS PAGE followed by western blot with 87874-1-RR (Phospho-ALK (Tyr1278) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: pervanadate treated Karpas-299 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87874-1-RR targets Phospho-ALK (Tyr1278) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: anaplastic lymphoma receptor tyrosine kinase
• Calculated Molecular Weight: 176 kDa
• Observed Molecular Weight: 80 kDa, 300 kDa
• GenBank Accession Number: NM_004304
• Gene Symbol: ALK
• Gene ID (NCBI): 238
• RRID: AB_3745801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UM73
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anaplastic lymphoma kinase was first described in 1994 as the NPM-ALK fusion protein that is expressed in the majority of anaplastic large-cell lymphomas. About 2/3rds of anaplastic large-cell lymphomas possess a balanced chromosomal translocation in which the entire nucle-ophosmin (NPM) gene on chromosome 5 is fused to the 3' portion of the ALK gene (including the entire intracellular segment with its protein kinase domain) on chromosome 2. This oncogenic ALK protein kinase is a chimeric protein created by a translocation between chromosomes(2;5)(p23;q35) that generates the NPM-ALK fusion protein. Phospho-ALK (Tyr1278) is a hallmark of ALK-driven malignancies. (PMID: 30400214)