Phospho-ASK1 (Ser83) Polyclonal antibody

Phospho-ASK1 (Ser83) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 28847-1-AP


ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Phospho-ASK1 (Ser83)

Tested Applications

Positive WB detected inHEK-293T cells, λ phosphatase treated HEK-293T cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28847-1-AP targets Phospho-ASK1 (Ser83) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase kinase kinase 5
Calculated Molecular Weight 155 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC054503
Gene Symbol MAP3K5
Gene ID (NCBI) 4217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. ASK1 is reportedly regulated by phosphorylation and dephosphorylation at four different phosphorylation sites. ASK1 activation is accompanied by phosphorylation of Thr845 and dephosphorylation of Ser83, Ser967, and Ser1034. STRAP actually decreased Thr838 (corresponding to Thr845 in mice) phosphorylation but increased Ser83 and Ser967 phosphorylation, an effect that would inactivate ASK1. (PMID: 19880523)


Product Specific Protocols
WB protocol for Phospho-ASK1 (Ser83) antibody 28847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols