Phospho-Beta Catenin (Ser33) Polyclonal antibody

• 

  WB analysis using 28772-1-AP

  Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28772-1-AP (Phospho-Beta Catenin (Ser33) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 28772-1-AP

  Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28772-1-AP (Phospho-Beta Catenin (Ser33) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-Beta Catenin (Ser33) Polyclonal Antibody for WB, ELISA

Cat No. 28772-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser33), PRO2286, β-Catenin

---

Tested Applications

• Positive WB detected in: Calyculin A treated PC-3 cells, Calyculin A treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

28772-1-AP targets Phospho-Beta Catenin (Ser33) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC058926
• Gene Symbol: Beta Catenin
• Gene ID (NCBI): 1499
• ENSEMBL Gene ID: ENSG00000168036
• RRID: AB_2923582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35222
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Catenin/β-catenin, also known as CTNNB1, is a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The N-terminal (1-49 amino acids) of β-catenin is the key region for its stability and the degradation by ubiquitin-proteasome system, and phosphorylation of β-catenin at the N-terminal Ser33, Ser37, Thr41, and Ser45 promotes its ubiquitination and degradation (PMID: 31930681). Phosphorylated β-catenin is targeted for ubiquitination-dependent proteolysis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Free Radic Biol Med; Fusobacterium nucleatum induces oxaliplatin resistance by inhibiting ferroptosis through E-cadherin/β-catenin/GPX4 axis in colorectal cancer; Authors - Bowen Li
human	WB	J Cancer; MPP7 mediates EMT via Wnt/β-catenin pathway to promote polarity changes in epithelial ovarian cancer cells; Authors - Chunlin Tao
human	WB	Exp Hematol Oncol; Fibronectin promotes tumor angiogenesis and progression of non-small-cell lung cancer by elevating WISP3 expression via FAK/MAPK/ HIF-1α axis and activating wnt signaling pathway; Authors - Fei Zhou
human	WB	Heliyon; MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling; Authors - Qingqing Chen