Phospho-Beta Catenin (Ser37) Polyclonal antibody

• 

  WB analysis using 28776-1-AP

  Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28776-1-AP (Phospho-Beta Catenin (Ser37) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-Beta Catenin (Ser37) Polyclonal Antibody for WB, ELISA

Cat No. 28776-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser37), Phospho-CTNNB1 (Ser37), PRO2286, β-Catenin

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Tested Applications

• Positive WB detected in: Calyculin A treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

28776-1-AP targets Phospho-Beta Catenin (Ser37) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 86-100 kDa
• GenBank Accession Number: BC058926
• Gene Symbol: Beta Catenin
• Gene ID (NCBI): 1499
• ENSEMBL Gene ID: ENSG00000168036
• RRID: AB_2935407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35222
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Catenin(β-catenin), also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-catenin is a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis.Deregulation of β-catenin activity is associated with multiple diseases including cancers. The N-terminal (1-49 amino acids) of β-catenin is the key region for its stability and the degradation by ubiquitin-proteasome system, and phosphorylation of β-catenin at the N-terminal Ser33, Ser37, Thr41, and Ser45 promotes its ubiquitination and degradation(PMID: 31930681).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Agric Food Chem; Ursolic Acid Suppresses Colorectal Cancer by Down-Regulation of Wnt/β-Catenin Signaling Pathway Activity; Authors - Hui Zhao
human	WB	Adv Sci (Weinh); Hepatocyte-Targeted Lipid Nanoparticle Delivery of HERC2 Plasmid Controls Drug-Induced Hepatotoxicity by Limiting β-Catenin-Regulated CYP2E1 Expression; Authors - Yunzhi Liu
human	WB	J Mol Histol; Activation of the WNT4/ β-catenin/FOXO1 pathway by PDK1 promotes cervical cancer metastasis and EMT process; Authors - Shidong Chen
human	WB	Front Pharmacol; Gut microbiota-derived metabolite phenylacetylglutamine inhibits the progression of prostate cancer by suppressing the Wnt/β-catenin signaling pathway; Authors - Jing Lv