Home
Products
Primary Antibodies
Recombinant Antibodies
Phospho-CAD (Ser1859) Recombinant monoclonal antibody, PBS Only (87337-2-PBS)

Phospho-CAD (Ser1859) Recombinant monoclonal antibody, PBS Only

Name: Phospho-CAD (Ser1859) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87337-2-PBS-100UG

Phospho-CAD (Ser1859) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87337-2-PBS

Clone No.252640C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CAD (Ser1859), p-CAD, p-CAD (Ser1859), Phospho-CAD, CAD

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIF/ICC

WB analysis using 87337-2-RR (same clone as 87337-2-PBS)
Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87337-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87337-2-PBS in a different storage buffer formulation.
WB analysis using 87337-2-RR (same clone as 87337-2-PBS)
Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87337-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87337-2-PBS in a different storage buffer formulation.
WB analysis using 87337-2-RR (same clone as 87337-2-PBS)
Non-treated MCF-7 cells and Rapamycin treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87337-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated MCF-7 cells and Rapamycin treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87337-2-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87337-2-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 87337-2-RR (same clone as 87337-2-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed Rapamycin treated MCF-7 cells using Phospho-CAD (Ser1859) antibody (87337-2-RR, Clone: 252640C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87337-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

87337-2-PBS targets Phospho-CAD (Ser1859) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Calculated Molecular Weight	2225 aa, 243 kDa
Observed Molecular Weight	250 kDa
GenBank Accession Number	NM_004341
Gene Symbol	CAD
Gene ID (NCBI)	790
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P27708
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides. Phospho-CAD (Ser1859) refers to the phosphorylated form of the multi-enzyme CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) on serine 1859. This modification is catalyzed by ribosomal protein S6 kinase 1 (S6K1) downstream of mTORC1 signaling and serves as a metabolic switch that converts CAD from its basal state into a highly active oligomer, thereby accelerating the first three rate-limiting steps of de novo pyrimidine synthesis. (PMID:25422319, PMID: 32974014)