Phospho-CDK1 (Tyr15) Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of HeLa using 82751-5-RR (same clone as 82751-5-PBS)

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 82751-5-RR (Phospho-CDK1 (Tyr15) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82751-5-PBS in a different storage buffer formulation.

Phospho-CDK1 (Tyr15) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 82751-5-PBS

Clone No.251130A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

cdc2 (Tyr15), CDK1 (Tyr15), p-cdc2, p-cdc2 (Tyr15), p-CDK1

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Product Information

82751-5-PBS targets Phospho-CDK1 (Tyr15) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cell division cycle 2, G1 to S and G2 to M
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC014563
• Gene Symbol: CDK1
• Gene ID (NCBI): 983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06493
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. Phospho-CDK1 (Tyr15) is the inactive, inhibited form of cyclin-dependent kinase 1 (CDK1) generated when the tyrosine 15 residue of CDK1's ATP-binding loop is phosphorylated by the kinases WEE1 and PKMYT1. This single phosphorylation event clamps the kinase in an inactive conformation, preventing premature onset of mitosis by blocking ATP binding and cyclin B1 activation. (PMID: 14645578)