Tested Applications
| Positive WB detected in | MMS treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29012-1-AP targets Phospho-CHEK2 (Thr68) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | CHK2 checkpoint homolog (S. pombe) |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC004207 |
| Gene Symbol | CHEK2 |
| Gene ID (NCBI) | 11200 |
| RRID | AB_2918230 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O96017 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-CHEK2 (Thr68) antibody 29012-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-CHEK2 (Thr68) Polyclonal antibody (Cat# 29012-1-AP) has 14 citations. These appear in journals including Research, Cell Death & Disease, EMBO Reports, Biochemical Pharmacology, International Journal of Molecular Sciences, Frontiers in Pharmacology, Chemical & Biological Interactions, Scientific Reports, Molecular Oncology, Medicine & Oncology, Journal of Agricultural and Food Chemistry, Cell Proliferation, and Frontiers in Oncology. Research fields span DNA damage response, DNA repair, oxidative stress, apoptosis, pyroptosis, and cancer biology (ovarian, lung, breast, colorectal, melanoma, pancreatic, and cholangiocarcinoma).
| Species | Application | Title |
|---|---|---|
Research (Wash D C) Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer | ||
Cell Death Dis PI3K/mTOR inhibitors promote G6PD autophagic degradation and exacerbate oxidative stress damage to radiosensitize small cell lung cancer | ||
Cell Prolif eIF3a-PPP2R5A-mediated ATM/ATR dephosphorylation is essential for irinotecan-induced DNA damage response. | ||
J Agric Food Chem Kaempferol Induces DNA Damage in Colorectal Cancer Cells by Regulating the MiR-195/miR-497-PFKFB4-Mediated Nonoxidative Pentose Phosphate Pathway | ||
EMBO Rep PARG inhibition reduces ssDNA levels and limits RPA loading upon replication fork collapse. |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars. The user tested it in Western Blot on HeLa cells at a 1:1000 dilution and noted that a 1:500 dilution works better for WB applications. Overall, the antibody performed well, with the only suggestion being to adjust the dilution for optimal results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Arnaud (Verified Customer) (08-04-2026) | Need to be at 1:500 dilution for WB
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