Phospho-CHEK2 (Thr68) Polyclonal antibody

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  WB analysis using 29012-1-AP

  Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29012-1-AP (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:1000 incubated at room temperature for 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-CHEK2 (Thr68) Polyclonal Antibody for WB, ELISA

Cat No. 29012-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

CDS1, CHEK2, CHK2, HuCds1, LFS2, PP1425, RAD53

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Tested Applications

• Positive WB detected in: MMS treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below

Product Information

29012-1-AP targets Phospho-CHEK2 (Thr68) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: CHK2 checkpoint homolog (S. pombe)
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC004207
• Gene Symbol: CHEK2
• Gene ID (NCBI): 11200
• RRID: AB_2918230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O96017
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; PI3K/mTOR inhibitors promote G6PD autophagic degradation and exacerbate oxidative stress damage to radiosensitize small cell lung cancer; Authors - Huan Deng
human	WB	Cell Prolif; eIF3a-PPP2R5A-mediated ATM/ATR dephosphorylation is essential for irinotecan-induced DNA damage response.; Authors - Chao Mei
human	WB	Front Oncol; ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway; Authors - Xin Wen
human	WB	Front Pharmacol; Synergistic effects of retinol and retinyl palmitate in alleviating UVB-induced DNA damage and promoting the homologous recombination repair in keratinocytes; Authors - Jiangming Zhong
human	WB	Mol Oncol; Olaparib synergy screen reveals Exemestane induces replication stress in triple-negative breast cancer; Authors - Nur Aininie Yusoh
human	WB	J Agric Food Chem; Kaempferol Induces DNA Damage in Colorectal Cancer Cells by Regulating the MiR-195/miR-497-PFKFB4-Mediated Nonoxidative Pentose Phosphate Pathway; Authors - Haili Wu