Tested Applications
| Positive WB detected in | Calyculin A treated HEK-293 cells, Calyculin A treated NIH/3T3 cells |
| Positive IHC detected in | human prostate cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
| Positive ChIP-qPCR detected in | Forskolin (30 μM, 1 h) HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81871-1-RR targets Phospho-CREB1 (Ser133) in WB, IHC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cAMP responsive element binding protein 1 |
| Calculated Molecular Weight | 341 aa, 35 kDa |
| Observed Molecular Weight | 35-46 kDa |
| GenBank Accession Number | BC010636 |
| Gene Symbol | CREB1 |
| Gene ID (NCBI) | 1385 |
| RRID | AB_2935430 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16220 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RR | Download protocol |
| IHC protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RR | Download protocol |
| WB protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-CREB1 (Ser133) Recombinant monoclonal antibody (5H5), catalog number 81871-1-RR, has 8 total citations. These appear in journals including Phytomedicine, EMBO Mol Med, Cell Mol Biol Lett, J Ethnopharmacol, Cell Oncol, PLoS Pathog, FASEB J, and BMC Neurosci. Research fields span stem cell biology, reproductive medicine, leukemia, pulmonary fibrosis, breast cancer, infectious disease, polycystic ovary syndrome, and post-stroke depression.
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs. | ||
Phytomedicine Chinese medicine Sheng Jing Decoction alleviates oligoasthenospermia by modulating PI3K/Akt and MAPK signaling pathways. | ||
EMBO Mol Med Extracellular cyclophilin A promotes B-cell acute lymphoblastic leukemia progression via MC2R. | ||
J Ethnopharmacol Multi-omics reveals the effects and mechanisms of Xinxue Granules against idiopathic pulmonary fibrosis through activating the cAMP/EPAC/CREB axis. | ||
Cell Oncol (Dordr) P2RY6(+) tumor-associated macrophages as a therapeutic target: suppressing tumor growth and enhancing chemosensitivity in triple-negative breast cancer. | ||
