Phospho-Caspase 9 (Ser196) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 80346-1-RR (same clone as 80346-1-PBS)

  Non-treated HEK-293 and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80346-1-RR (Phospho-Caspase 9 (Ser196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 80346-1-PBS in a different storage buffer formulation.

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  WB analysis using 80346-1-RR (same clone as 80346-1-PBS)

  Non-treated Jurkat and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80346-1-RR (Phospho-Caspase 9 (Ser196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 80346-1-PBS in a different storage buffer formulation.

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  WB analysis using 80346-1-RR (same clone as 80346-1-PBS)

  Non-treated NIH/3T3 and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80346-1-RR (Phospho-Caspase 9 (Ser196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 80346-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80346-1-RR (same clone as 80346-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-Caspase 9 (Ser196) antibody (80346-1-RR, Clone: 3P16 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80346-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 80346-1-RR (same clone as 80346-1-PBS)

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-Caspase 9 (Ser196) Recombinant antibody (80346-1-RR, Clone:3P16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80346-1-PBS in a different storage buffer formulation.

Phospho-Caspase 9 (Ser196) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 80346-1-PBS

Clone No.3P16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Caspase 9 (Ser196), p Caspase 9, p Caspase 9 (Ser196), p Caspase 9 Ser196, p-Caspase 9

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Product Information

80346-1-PBS targets Phospho-Caspase 9 (Ser196) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: caspase 9, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa, 35 kDa
• GenBank Accession Number: BC002452
• Gene Symbol: Caspase 9
• Gene ID (NCBI): 842
• RRID: AB_2918890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55211
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46kDa precursor protein which can be cleaved into a 35kDa subunit and a 11kDa subunit. The phosphorylated type can be detected at 55kDa and 35kDa. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It can be phosphorylated by PKB/AKT1 at Ser196, this modification will downregulate its activity and decrease apoptosis. Akt phosphorylation site found in human caspase 9 is absent in mouse caspase 9.It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507) In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].