Tested Applications
| Positive WB detected in | Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells |
| Positive IF/ICC detected in | Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28803-1-AP targets Phospho-Chk1 (Ser345) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | CHK1 checkpoint homolog (S. pombe) |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC004202 |
| Gene Symbol | Chk1 |
| Gene ID (NCBI) | 1111 |
| RRID | AB_2918206 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14757 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-Chk1 (Ser345) antibody 28803-1-AP | Download protocol |
| WB protocol for Phospho-Chk1 (Ser345) antibody 28803-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Transl Oncol The ATR inhibitor VE-821 increases the sensitivity of gastric cancer cells to cisplatin | ||
Biochem Biophys Res Commun JMJD5 promotes chemotherapy sensitivity of cisplatin in NSCLC by enhancing DNA damage. | ||
Autophagy CCPG1-mediated reticulophagy promotes tumorigenesis and cisplatin resistance in bladder cancer. | ||
Drug Resist Updat RAD1 promotes oxaliplatin resistance in gastric cancer by reinforcing NRF2-driven antioxidant defense and DDR checkpoint signaling. | ||
Cell Biol Int RPL27 Promotes Gastric Cancer Progression by Enhancing Oxidative Phosphorylation Via FMC1. |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Arnaud (Verified Customer) (08-04-2026) | Some aspecific bands at the same size
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