Phospho-Chk1 (Ser345) Polyclonal antibody

Phospho-Chk1 (Ser345) Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse and More (1)





Cat no : 28803-1-AP


CHEK1, CHK1, Phospho-CHK1 (Ser345)

Tested Applications

Positive WB detected inCalyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells
Positive IF detected inCalyculin A treated HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

28803-1-AP targets Phospho-Chk1 (Ser345) in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated Molecular Weight 54 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC004202
Gene Symbol CHEK1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)


Product Specific Protocols
WB protocol for Phospho-Chk1 (Ser345) antibody 28803-1-APDownload protocol
IF protocol for Phospho-Chk1 (Ser345) antibody 28803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Transl Oncol

The ATR inhibitor VE-821 increases the sensitivity of gastric cancer cells to cisplatin

Authors - Haochen Su


Epiberberine induced p53/p21-dependent G2/M cell cycle arrest and cell apoptosis in gastric cancer cells by activating γ-aminobutyric acid receptor- β3

Authors - Mengmeng Li