Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29715-1-AP targets Phospho-Cofilin (Ser3) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cofilin 1 (non-muscle) |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC012318 |
| Gene Symbol | Cofilin |
| Gene ID (NCBI) | 1072 |
| RRID | AB_2935421 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23528 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-Cofilin (Ser3) antibody 29715-1-AP | Download protocol |
| WB protocol for Phospho-Cofilin (Ser3) antibody 29715-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-Cofilin (Ser3) Polyclonal antibody (Cat# 29715-1-AP) has 1 total citation. It appears in BMC Cardiovasc Disord (2025), where it was used for Western blot in human samples to investigate AMPK-mediated cytoskeletal dynamics in lower extremity varicose vein development. The associated research field is cardiovascular disease, specifically involving actin cytoskeleton regulation and vascular pathology.
| Species | Application | Title |
|---|---|---|
BMC Cardiovasc Disord Mechanism of adenosine monophosphate-activated protein kinase promoting lower extremity varicose veins development via cytoskeletal dynamics |
Reviews
What customers say
The product received one 5-star review from a researcher who used it for Western Blot on glioblastoma (GBM) cells at a 1:800 dilution in 3% BSA. The reviewer reported that it worked well, confirming reliable performance for this application. Overall, the feedback is positive, with the user satisfied with the antibody's performance under their specific experimental conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Udesh (Verified Customer) (03-05-2024) | Worked well for WB of glioma cells at 1:800 in 3% BSA
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