Phospho-EGFR (Tyr1092) Polyclonal antibody

Phospho-EGFR (Tyr1092) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30278-1-AP

Synonyms

EGFR, ERBB, ERBB1, HER1, mENA, Phospho-EGFR (Tyr1068), Phospho-EGFR (Tyr1092), PIG61, Proto oncogene c ErbB 1

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Validation Data Gallery

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  WB analysis using 30278-1-AP

  Non-treated HeLa and EGF treated HeLa cells were subjected to SDS PAGE followed by western blot with 30278-1-AP (Phospho-EGFR (Tyr1092) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

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Tested Applications

• Positive WB detected in: EGF treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30278-1-AP targets Phospho-EGFR (Tyr1092) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 1210 aa, 134 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC094761
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1092 as EGFR Tyr1068, the site where the signaling peptide is removed.