Phospho-EGFR (Tyr1110) Recombinant monoclonal antibody

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• Phospho-EGFR (Tyr1110) Recombinant monoclonal antibody (87084-2-RR)

Phospho-EGFR (Tyr1110) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 87084-2-RR

Clone No.251800D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

p-EGF Receptor, p-EGF Receptor (Tyr1086), p-EGFR (Tyr1086), p-EGFR (Tyr1110), p-EGFR Y1086

• 

  WB analysis using 87084-2-RR

  Non-treated A431 cells and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 87084-2-RR (Phospho-EGFR (Tyr1110) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Schematic diagram of the phosphorylation sites of EGFR.

  In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 87084-2-RR

  Immunofluorescent analysis of (4% PFA) fixed EGF treated A431 cells using Phospho-EGFR (Tyr1110) antibody (87084-2-RR, Clone: 251800D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of A431 using 87084-2-RR

  1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.13 ug Phospho-EGFR (Tyr1110) Recombinant monoclonal antibody (87084-2-RR, Clone:251800D1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: EGF treated A431 cells
• Positive IF/ICC detected in: EGF treated A431 cells
• Positive FC (Intra) detected in: EGF treated100ng/ml ,30min A431 cells

This antibody, also known as EGFR Tyr1086, is the site where the signal peptide is removed.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87084-2-RR targets Phospho-EGFR (Tyr1110) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 1210 aa, 134 kDa
• Observed Molecular Weight: 175 kDa
• GenBank Accession Number: BC094761
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• RRID: AB_3745324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00533
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Phospho-EGFR (Tyr1110) is the phosphorylated, active form of the epidermal growth factor receptor (EGFR) at tyrosine 1110 within the C-terminal tail. This modification is catalyzed by EGFR itself or SRC-family kinases upon ligand (EGF, TGF-α) binding or oxidative stress. Phosphorylation at Tyr1110 creates a docking site for SH2-domain-containing proteins, propagating downstream signals through the RAS-RAF-MEK-ERK and PI3K-AKT pathways. Some scientists call EGFR Tyr1110 as EGFR Tyr1086, the site where the signaling peptide is removed.