Phospho-EGFR (Tyr1172) Recombinant monoclonal antibody

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  WB analysis of A431 using 87164-1-RR

  Non-treated A431 cells, Serum-free treated and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 87164-1-RR (Phospho-EGFR (Tyr1172) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.

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  Schematic diagram of the phosphorylation sites of EGFR.

  In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.

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  IF Staining of A431 using 87164-1-RR

  Immunofluorescent analysis of (4% PFA) fixed EGF treated A431 cells using Phospho-EGFR (Tyr1172) antibody (87164-1-RR, Clone: 252172E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-EGFR (Tyr1172) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87164-1-RR

Clone No.252172E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EGFR (phospho Y1172), EGFR (Tyr1172), p-EGF Receptor, p-EGF Receptor (Tyr1148), p-EGFR

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Tested Applications

• Positive WB detected in: EGF treated A431 cells, Serum-free treated and EGF treated A431 cells
• Positive IF/ICC detected in: EGF treated A431 cells

This antibody, also known as EGFR Tyr1148, is the site where the signal peptide is removed.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87164-1-RR targets Phospho-EGFR (Tyr1172) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 1210 aa, 134 kDa
• Observed Molecular Weight: 175 kDa
• GenBank Accession Number: BC094761
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00533
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Phospho-EGFR (Tyr1172) is a phosphorylated form of the epidermal growth factor receptor (EGFR), which is a transmembrane glycoprotein receptor that belongs to the ErbB family of receptor tyrosine kinases (RTKs). This phosphorylation event is significant as it activates downstream signaling cascades that regulate various cellular processes, including cell growth, proliferation, and survival. Some scientists call EGFR Tyr1172 as EGFR Tyr1148, the site where the signaling peptide is removed.