Phospho-ErbB3/HER3 (Tyr1197) Recombinant monoclonal antibody, PBS Only

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  WB analysis of T-47D using 87133-1-RR (same clone as 87133-1-PBS)

  Non-treated T-47D cells and human Neuregulin-1 treated T-47D cells were subjected to SDS PAGE followed by western blot with 87133-1-RR (Phospho-ErbB3/HER3 (Tyr1197) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87133-1-PBS in a different storage buffer formulation.

Phospho-ErbB3/HER3 (Tyr1197) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87133-1-PBS

Clone No.252296B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ErbB 3 (phospho Y1197), Her3/ErbB3(phospho-Tyr1197), p-ErbB3/HER3, p-ErbB3/HER3 (Tyr1197), p-ErbB3/HER3(Tyr1197)

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Product Information

87133-1-PBS targets Phospho-ErbB3/HER3 (Tyr1197) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: NM_001982
• Gene Symbol: ERBB3
• Gene ID (NCBI): 2065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21860
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. Phospho-ErbB3/HER3 (Tyr1197) is a phosphorylated form of the epidermal growth factor receptor 3 (EGFR3), also known as HER3. HER3 lacks tyrosine kinase activity itself, but becomes phosphorylated at tyrosine residues after forming heterodimers with other ErbB proteins such as HER2. The HER2/HER3 dimer is a potent activator of growth signaling pathways including MAPK. This phosphorylation event is involved in signal transduction pathways related to cell growth, proliferation, and survival. (PMID: 35625984)