Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84564-6-RR (same clone as 84564-6-PBS)

  T-47D cell lysates were subjected to SDS PAGE followed by western blot with 84564-6-RR (Phospho-Estrogen Receptor α (Ser118) antibody) blocked with BSA only, Phospho-Estrogen Receptor α (Ser118) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84564-6-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 84564-6-RR (same clone as 84564-6-PBS)

  1X10^6 MCF-7 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody (84564-6-RR, Clone:250278F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 84564-6-PBS in a different storage buffer formulation.

Phospho-Estrogen Receptor α (Ser118) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 84564-6-PBS

Clone No.250278F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Estrogen Receptor α (Ser118), p Estrogen Receptor α, p-ERS1, p-ERS1 (Ser118), p-Estrogen Receptor α

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Product Information

84564-6-PBS targets Phospho-Estrogen Receptor α (Ser118) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: estrogen receptor 1
• Calculated Molecular Weight: 595 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC128573
• Gene Symbol: ER
• Gene ID (NCBI): 2099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P03372
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Estrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633)