Phospho-Ezrin (Tyr354) Recombinant monoclonal antibody

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• Phospho-Ezrin (Tyr354) Recombinant monoclonal antibody (88246-2-RR)

Phospho-Ezrin (Tyr354) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88246-2-RR

Clone No.260442B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Ezrin (Tyr353), Ezrin (Tyr354), p-Ezrin, p-Ezrin (Tyr353), p-Ezrin (Tyr354)

• 

  WB analysis using 88246-2-RR

  Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 88246-2-RR (Phospho-Ezrin (Tyr354) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (HRP-80762) antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: EGF treated A431 cells

Ezrin phosphorylates at Tyr354, sometimes called Tyr353.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88246-2-RR targets Phospho-Ezrin (Tyr354) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ezrin
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: NM_001111077
• Gene Symbol: Ezrin
• Gene ID (NCBI): 7430
• ENSEMBL Gene ID: ENSG00000092820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15311
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. Phospho-Ezrin (Tyr354), also annotated as Tyr353 in early literature, is a tyrosine-phosphorylated active form of ERM scaffold protein Ezrin encoded by the EZR gene. Triggered by HGF receptor tyrosine kinase, this modification unlocks Ezrin's autoinhibited conformation and enables high-affinity binding to the p85 regulatory subunit of PI3K. It sustains PI3K/Akt survival signaling, suppresses epithelial apoptosis, and drives cytoskeleton remodeling for cell migration and tubulogenesis. Unique from Thr567-phosphorylated Ezrin at cell membranes, p-Ezrin (Tyr354) translocates into nuclei in osteosarcoma and lung cancer. Its elevated nuclear expression correlates with tumor invasion, metastasis, and poor clinical prognosis, marking it a promising malignant progression biomarker. (PMID: 10377409, 20348881)