Phospho-FRS2 (Tyr436) Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of NIH/3T3 using 85242-1-RR (same clone as 85242-1-PBS)

  Non-treated NIH/3T3 cells and bFGF (HZ-1285) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85242-1-RR (Phospho-FRS2 (Tyr436) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 85242-1-PBS in a different storage buffer formulation.

Phospho-FRS2 (Tyr436) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85242-1-PBS

Clone No.242134H8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

P FRS2 (Tyr436), P-FRS2 (Tyr436), Phos FRS2 (Tyr436), Phos-FRS2 (Tyr436), Phospho-FRS2-α (Tyr436)

---

Product Information

85242-1-PBS targets Phospho-FRS2 (Tyr436) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: fibroblast growth factor receptor substrate 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC021562
• Gene Symbol: FRS2
• Gene ID (NCBI): 10818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WU20
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fibroblast growth factor substrate 2 (FRS2), a lipid-anchored docking protein that is phosphorylated upon activation of FGFR, is critical for recruitment of downstream signaling molecules and links the FGFRs to the Ras/Mek/Erk pathway and the PI3-Kinase/Akt pathway. Studies have found that the phosphotyrosine binding domain of FRS-2 directly binds the Trk receptors at the same phosphotyrosine residue that binds the signaling adapter Shc, suggesting a model in which competitive binding between FRS-2 and Shc regulates differentiation versusproliferation. (PMID: 19053057, PMID: 10092678)