Phospho-Filamin A (Ser2152) Recombinant monoclonal antibody, PBS Only

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  WB analysis of A431 using 87513-1-RR (same clone as 87513-1-PBS)

  Non-treated A431 cells and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 87513-1-RR (Phospho-Filamin A (Ser2152) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87513-1-PBS in a different storage buffer formulation.

Phospho-Filamin A (Ser2152) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87513-1-PBS

Clone No.252855H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

p-Filamin A (S2152), p-Filamin A Ser2152, p-FLNA Ser2152, Phospho Filamin A (Ser2152), Phospho FLNA (Ser2152)

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Product Information

87513-1-PBS targets Phospho-Filamin A (Ser2152) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: filamin A, alpha (actin binding protein 280)
• Calculated Molecular Weight: 2647 aa, 280 kDa
• Observed Molecular Weight: 280 kDa
• GenBank Accession Number: NM_001110556
• Gene Symbol: FLNA
• Gene ID (NCBI): 2316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21333
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network. Phospho-Filamin A (Ser2152) is a phosphorylated form of Filamin A (FLNA), which is a large actin-binding protein that plays a crucial role in organizing the cytoskeleton and transducing signals from cell membrane to the cytoskeleton. This phosphorylation event is associated with various cellular functions, including cytoskeletal remodeling, cell migration, and focal adhesion formation. (PMID:29931263)