Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82371-5-RR (same clone as 82371-5-PBS)

  HEK-293 cells and NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82371-5-RR (Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) antibody) blocked with BSA only, Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) peptide or non-Phospho peptide at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 82371-5-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 82371-5-RR (same clone as 82371-5-PBS)

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Recombinant monoclonal antibody (82371-5-RR, Clone:242663E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 90% MeOH . This data was developed using the same antibody clone with 82371-5-PBS in a different storage buffer formulation.

Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 82371-5-PBS

Clone No.242663E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

P-GSK3A (Tyr279), P-GSK3B (Tyr216), Phos-GSK3 beta (Tyr216), Phos-GSK3A (Tyr279), Phos-GSK3B (Tyr216)

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Product Information

82371-5-PBS targets Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC000251
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49841
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. GSK3 beta is activated by phosphorylation of the tyrosine 216 residue (Tyr216) located in the kinase domain and inactivated by phosphorylation of the amino-terminal serine 9 residue (Ser9). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704,PMID: 24722055)