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Phospho-HER2/ErbB2 (Tyr1196) Recombinant monoclonal antibody, PBS Only (87205-1-PBS)

Phospho-HER2/ErbB2 (Tyr1196) Recombinant monoclonal antibody, PBS Only

Name: Phospho-HER2/ErbB2 (Tyr1196) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87205-1-PBS-100UG

Phospho-HER2/ErbB2 (Tyr1196) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87205-1-PBS

Clone No.252284B12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

HER2/ErbB2 (phospho-Tyr1196), p-ErbB2 Tyr1196, p-HER2 Tyr1196, p-HER2/ErbB2, p-HER2/ErbB2 (Tyr1196)

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWB

WB analysis of T-47D using 87205-1-RR (same clone as 87205-1-PBS)
Non-treated T-47D cells and human Neuregulin-1 treated T-47D cells were subjected to SDS PAGE followed by western blot with 87205-1-RR (Phospho-HER2/ErbB2 (Tyr1196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87205-1-PBS in a different storage buffer formulation.
×
Non-treated T-47D cells and human Neuregulin-1 treated T-47D cells were subjected to SDS PAGE followed by western blot with 87205-1-RR (Phospho-HER2/ErbB2 (Tyr1196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87205-1-PBS in a different storage buffer formulation.
WB analysis using 87205-1-RR (same clone as 87205-1-PBS)
Non-treated MCF-7 cells and human Neuregulin-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87205-1-RR (Phospho-HER2/ErbB2 (Tyr1196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87205-1-PBS in a different storage buffer formulation.
×
Non-treated MCF-7 cells and human Neuregulin-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87205-1-RR (Phospho-HER2/ErbB2 (Tyr1196) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87205-1-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87205-1-PBS targets Phospho-HER2/ErbB2 (Tyr1196) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Calculated Molecular Weight	1255 aa, 138 kDa
Observed Molecular Weight	185 kDa
GenBank Accession Number	NM_004448
Gene Symbol	HER2/ErbB2
Gene ID (NCBI)	2064
RRID	AB_3745406
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P04626
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr1196) is a phosphorylated form of the HER2 protein, which is a receptor tyrosine kinase that plays a critical role in cell growth, proliferation, and survival. The phosphorylation of HER2 at Tyr1196 is associated with the activation of downstream signaling pathways. In breast cancer, HER2 is overexpressed and contributes to aggressive tumor growth and poor prognosis. Overexpression of Her2/ErbB2/Neu in cancer is often correlated with recurrent distant metastasis, although the mechanism still remains largely elusive. Tyr1139/Tyr1196 are autonomously phosphorylated, when Her2 is overexpressed. (PMID: 21966491)