Phospho-HSP27 (Ser78) Polyclonal antibody

Phospho-HSP27 (Ser78) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB

Conjugate

Unconjugated

Cat no : 28900-1-AP

Synonyms

DMPK binding protein, heat shock 27kDa protein 2, Heat shock protein beta 2, Hs.78846, Hsp B1, HSP27, LOH11CR1K, MKBP, Phospho-HSP27 (Ser78)



Tested Applications

Positive WB detected inTNF-alpha treated HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

28900-1-AP targets Phospho-HSP27 (Ser78) in WB applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name heat shock 27kDa protein 1
Calculated molecular weight 19-23 kDa
Observed molecular weight27-30 kDa
GenBank accession numberBC012768
Gene symbol HSPB1
Gene ID (NCBI) 3315
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)

Protocols

Product Specific Protocols
WB protocol for Phospho-HSP27 (Ser78) antibody 28900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols