Phospho-Histone H2A (Thr120) Recombinant monoclonal antibody

• 

  WB analysis of HeLa using 83041-8-RR

  Nocodazole and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 83041-8-RR (Phospho-Histone H2A (Thr120) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.

Phospho-Histone H2A (Thr120) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83041-8-RR

Clone No.250365B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Histone H2A (Thr120), p Histone H2A, p Histone H2A (Thr120), p Histone H2A Thr120, p-Histone H2A

---

Tested Applications

• Positive WB detected in: Nocodazole and Calyculin A treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83041-8-RR targets Phospho-Histone H2A (Thr120) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H2ae
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC093836
• Gene Symbol: HIST1H2AE
• Gene ID (NCBI): 3012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04908
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospho-Histone H2A (Thr120) refers to the phosphorylation of threonine 120 on histone H2A, a modification that is increasingly recognized for its roles in chromatin organization, gene regulation, and disease development, particularly in oncogenesis and bone remodeling. The phosphorylation is predominantly associated with gene silencing. High levels of H2AT120p correlate with increased cell proliferation, tumor growth, and poor prognosis, underscoring its role as an oncogenic signal.