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Phospho-Histone H3 (Thr3) Recombinant monoclonal antibody, PBS Only (84739-2-PBS)

Phospho-Histone H3 (Thr3) Recombinant monoclonal antibody, PBS Only

Name: Phospho-Histone H3 (Thr3) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 84739-2-PBS-100UG

Phospho-Histone H3 (Thr3) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA, ChIP-qPCR

Cat No. 84739-2-PBS

Clone No. 241897D9

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA, ChIP-qPCR

Synonyms

H3, H3 P-T3, H3T3, H3T3Ph, Histone H3 (Thr3)

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

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Filter: AllWBFC (Intra)ChIP-qPCR

WB analysis of HeLa using 84739-2-RR (same clone as 84739-2-PBS)
Calyculin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84739-2-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84739-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Calyculin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84739-2-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84739-2-PBS in a different storage buffer formulation.
FC experiment of NIH/3T3 using 84739-2-RR (same clone as 84739-2-PBS)
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR, Clone:241897D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84739-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-2-RR, Clone:241897D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84739-2-PBS in a different storage buffer formulation.
ChIP experiment of HeLa using 84739-2-RR (same clone as 84739-2-PBS)
Chromatin was prepared from HeLa cells treated with Nocodazole (100 ng/ml) for 16 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Phospho-Histone H3 (Thr3) (84739-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84739-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Chromatin was prepared from HeLa cells treated with Nocodazole (100 ng/ml) for 16 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Phospho-Histone H3 (Thr3) (84739-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84739-2-PBS in a different storage buffer formulation.

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Product Information

84739-2-PBS targets Phospho-Histone H3 (Thr3) in WB, FC (Intra), Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	histone cluster 1, H3a
Observed Molecular Weight	17 kDa
GenBank Accession Number	BC066245
Gene Symbol	HIST1H3A
Gene ID (NCBI)	8350
RRID	AB_3672124
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P68431
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.