Tested Applications
| Positive WB detected in | LPS treated THP-1 cells, LPS treated RAW 264.7 cells, THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29528-1-AP targets Phospho-IRF3 (Ser396) in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | interferon regulatory factor 3 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 45-55 kDa |
| GenBank Accession Number | BC009395 |
| Gene Symbol | IRF3 |
| Gene ID (NCBI) | 3661 |
| RRID | AB_2935415 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14653 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interferons (IFNs) are a large family of multifunctional secreted proteins involved in antiviral defense, cell growth regulation, and immune activation(PMID: 18440553). A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. IRF-3 is activated through phosphorylation by the kinases IKKɛ and/or TBK1. Phosphorylation at Ser396 is essential for IRF-3 activation. After cytoplasmic IRF-3 is phosphorylated at Ser386 and Ser396, phosphorylated IRF-3 translocates into the nucleus. This phosphorylated IRF-3 activates IFN and IFN-responsive genes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-IRF3 (Ser396) antibody 29528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-IRF3 (Ser396) Polyclonal antibody (Cat# 29528-1-AP) has 76 citations across journals including Signal Transduction and Targeted Therapy, Cancer Research, Advanced Science, Cell Death & Differentiation, Biomaterials, International Journal of Biological Sciences, Acta Biomaterialia, Journal of Translational Medicine, Frontiers in Immunology, PLoS Pathogens, and Veterinary Research. Research fields span cGAS-STING signaling, innate immunity, cancer immunotherapy, antiviral responses, neuroinflammation, ferroptosis, and viral pathogenesis.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation | ||
Cancer Res An Immune Subtype Classification System Enables the Development of Strategies to Predict and Enhance Immunotherapy Responses in Colorectal Cancer | ||
Cancer Res CDK4/6 Alters TBK1 Phosphorylation to Inhibit the STING Signaling Pathway in Prostate Cancer | ||
Exp Hematol Oncol Overexpression of ESYT3 improves radioimmune responses through activating cGAS-STING pathway in lung adenocarcinoma | ||
ACS Nano Biomimetic Nanoregulators Mediated Tyrosine Hydroxylase mRNA and Stimulator of Interferon Genes Antagonist Codelivery for Synergistic Therapy on Parkinson's Disease. | ||
J Nanobiotechnology Platelet hitchhiking vascular-disrupting agents for self-amplified tumor-targeting therapy |
Reviews
What customers say
Marina Rodriguez gave a 5-star rating for this antibody used in Western Blot on triple negative breast cancer cell lines. She used a 1:1000 dilution with overnight incubation and Ponceau staining as a total protein loading control. The review was posted on December 20, 2025, using lot 00194069.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marina (Verified Customer) (12-20-2025) | 1:1000 dilution, overnight incubation. Ponceau staining was used as total protein loading control.
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