Tested Applications
| Positive WB detected in | THP-1 cells |
| Positive IF/ICC detected in | LPS treated THP-1 cells |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80519-2-RR targets Phospho-IRF3 (Ser396) in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | interferon regulatory factor 3 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 55-65 kDa |
| GenBank Accession Number | BC009395 |
| Gene Symbol | IRF3 |
| Gene ID (NCBI) | 3661 |
| RRID | AB_3670478 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14653 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-IRF3 (Ser396) antibody 80519-2-RR | Download protocol |
| WB protocol for Phospho-IRF3 (Ser396) antibody 80519-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The human recombinant antibody 80519-2-RR (anti-STING) has 14 citations across 12 journals, including Acta Pharm Sin B, Phytomedicine, Cancer Immunol Res, Int Immunopharmacol, BMC Microbiol, and Adv Sci. Research fields span cGAS-STING innate immunity, antiviral signaling, cancer immunotherapy, inflammatory bowel disease, fatty liver/NASH, pyroptosis, and PANoptosis. Applications include WB and IHC in human, mouse, and chicken models.
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B A new approach to discovering a broad-spectrum anti-respiratory virus drug: Tubeimoside II modulates host factor PACT to potentiate the RIG-I antiviral pathway. | ||
Phytomedicine Brucea javanica oil emulsion ameliorates ulcerative colitis by upregulating Deoxyribonuclease 2 to suppress cytosolic DNA-sensing signaling. | ||
Cancer Immunol Res FAM135B Deficiency Inhibits Cytotoxic T-cell Activity in Triple-Negative Breast Cancer by Blocking the IFI16-Dependent STING Pathway. | ||
Am J Chin Med Paeoniflorin Alleviates Metabolic Dysfunction-Associated Fatty Liver Disease by Targeting STING-Mediated Pyroptosis via Inhibiting the NLRP3 Inflammasome. | ||
Biomed Pharmacother Simultaneous activation of apoptosis and cGAS-STING pathway with a mitochondria-targeted dehydrocholic acid compound for enhanced hepatocellular carcinoma chemoimmunotherapy. | ||
Colloids Surf B Biointerfaces STING-engineered biomimetic hybrid membrane nanovaccine for enhanced immunotherapy of cervical cancer. |
Reviews
What customers say
The product has one review. The reviewer used it for Western Blot at a 1:2000 dilution on HeLa cells and rated it 5 stars, describing it as a "good antibody." Overall, the feedback is positive, indicating reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Arnaud (Verified Customer) (08-04-2026) | Good antibody
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