Phospho-IkB Alpha (Ser32/36) Recombinant antibody

Phospho-IkB Alpha (Ser32/36) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

1G21

Cat no : 82349-1-RR

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA, Phospho-IkB Alpha (Ser32/36)

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Validation Data Gallery

• 

  WB analysis of HeLa using 82349-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha-tubulin antibody as loading control.

• 

  WB analysis using 82349-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 82349-1-RR

  PMA treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Calyculin A treated HeLa cells, PMA treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

82349-1-RR targets Phospho-IkB Alpha (Ser32/36) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004983
• Gene Symbol: NFKBIA
• Gene ID (NCBI): 4792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha). (PMID: 16904979, PMID: 28990531 PMID: 10455908)

Publications

Species	Application	Title
human	WB	Transl Oncol; Endogenous hydrogen sulfide inhibition suppresses tumor growth by promoting apoptosis and pyroptosis in esophageal cancer cells; Authors - Hong-Gang Wang
rat	WB	Phytomedicine; Ganoderma lucidum polysaccharide peptide (GLPP) attenuates rheumatic arthritis in rats through inactivating NF-κB and MAPK signaling pathways; Authors - Meng Meng
human	WB	Mol Immunol; Catapol attenuates the aseptic inflammatory response to hepatic I/R injury in vivo and in vitro by inhibiting the HMGB1/TLR-4/NF-κB signaling pathway via the microRNA-410-3p; Authors - Zan Jie Feng
human	WB	Am J Physiol Cell Physiol; Inducible nitric oxide synthase activity mediates TNF-α-induced endothelial cell dysfunction; Authors - Chen Liu
human,rat	WB	J Ethnopharmacol; The Terminalia chebula Retz extract treats hyperuricemic nephropathy by inhibiting TLR4/MyD88/NF-κB axis; Authors - Hao Liu
human,mouse	WB	Int J Biol Macromol; In vitro and in vivo immunomodulatory activity of acetylated polysaccharides from Cyclocarya paliurus leaves; Authors - Yang Zhang