Phospho-JUN (Ser63) Recombinant monoclonal antibody

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• Phospho-JUN (Ser63) Recombinant monoclonal antibody (84147-1-RR)

Phospho-JUN (Ser63) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA, ChIP-qPCR

Cat No. 84147-1-RR Copied!

Clone No. 240965B7

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA, ChIP-qPCR

Synonyms

c-Jun (Ser63), P-cJun, p-c-Jun, p-c-Jun (Ser63), p-c-Jun(Ser63)

• 

  WB analysis using 84147-1-RR

  Non-treated NIH/3T3 cells, Anisomycin treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84147-1-RR (Phospho-JUN (Ser63) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of A431 using 84147-1-RR

  1X10^6 A431 cells untreated (dashed lines) or treated with UV which intracellularly stained with 0.13 ug Phospho-JUN (Ser63) Recombinant monoclonal antibody (84147-1-RR, Clone:240965B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  ChIP experiment of PC-12 using 84147-1-RR

  Chromatin was prepared from PC-12 cells starved overnight and treated with Human β-Nerve Growth Factor (hβ-NGF) (50 ng/ml, 2 hr). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-JUN (Ser63) (84147-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Anisomycin treated NIH/3T3 cells, UV treated NIH/3T3 cells
• Positive FC (Intra) detected in: UV treated A431 cells
• Positive ChIP-qPCR detected in: hβ-NGF (50 ng/ml, 2 hr) PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84147-1-RR targets Phospho-JUN (Ser63) in WB, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: jun oncogene
• Calculated Molecular Weight: 331 aa, 36 kDa
• Observed Molecular Weight: 40-48 kDa
• GenBank Accession Number: BC068522
• Gene Symbol: JUN
• Gene ID (NCBI): 3725
• RRID: AB_3671710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05412
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)

Publications

What published studies show

The Phospho-JUN (Ser63) Recombinant monoclonal antibody (240965B7), catalog number 84147-1-RR, has 1 citation. It appears in the journal Gene (Impact Factor 2.7), in a 2026 study by Huilong Fang examining PDIA2's role in lung cancer progression via JNK and caspase-3 signaling. The associated research field is oncology, specifically lung cancer and MAPK/caspase signaling pathways.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Gene; PDIA2 promotes progression of lung cancer by regulating JNK and caspase-3 signaling pathway.; Authors - Huilong Fang