Tested Applications
| Positive WB detected in | nocodazole treated A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28930-1-AP targets Phospho-MEK1 (Thr292) in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase 1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC139729 |
| Gene Symbol | MEK1 |
| Gene ID (NCBI) | 5604 |
| ENSEMBL Gene ID | ENSG00000169032 |
| RRID | AB_2918216 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02750 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis. MEK1 has 2 isoforms produced by alternative splicing with the molecular weight of 43 kDa and 41 kDa. MEK1 may form a heterodimer with Mek2.(PMID:21144847,19219045).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MEK1 (Thr292) antibody 28930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-MEK1 (Thr292) Polyclonal antibody (Cat# 28930-1-AP) has 23 citations across journals including Cell Death & Disease, Cell Reports, International Journal of Biological Sciences, Journal of Translational Medicine, Scientific Reports, Cell Signaling, iScience, and Ecotoxicology and Environmental Safety. Research fields span MAPK/ERK signaling in various cancers (TNBC, HCC, gastric, thyroid, ovarian, AML), pulmonary fibrosis, lung injury, osteoporosis, neuroinflammation, wound repair, and toxicology. Most applications are Western blot in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis PLSCR1 drives chemoresistance in TNBC via METTL3/IGF2BP3-mediated mRNA stabilization and EGFR-MAPK pathway activation. | ||
Int J Biol Sci miR-34a-5p functions as a tumor suppressor in head and neck squamous cell cancer progression by targeting Flotillin-2. | ||
J Transl Med Multiomic analysis of monocyte-derived alveolar macrophages in idiopathic pulmonary fibrosis | ||
Int J Biol Macromol Promotion of the genipin crosslinked chitosan-fiber hydrogel loaded with sustained release of clemastine fumarate in diabetic wound repair | ||
Cell Rep Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism | ||
World J Gastroenterol TATA-box-binding protein-associated factor 15 is a novel biomarker that promotes cell proliferation and migration in gastrointestinal stromal tumor |
