Phospho-MOBKL1B (Thr12) Polyclonal antibody

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  WB analysis using 29027-1-AP

  Phosphatase inhibitor treated and λ phosphatase treated PC-12 cells were subjected to SDS PAGE followed by western blot with 29027-1-AP (Phospho-MOBKL1B (Thr12) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of U2OS using 29027-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated U2OS cells using Phospho-MOBKL1B (Thr12) antibody (29027-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-MOBKL1B (Thr12) Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29027-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

MOBKL1B (Thr12), p MOBKL1B , p MOBKL1B (Thr12), p MOBKL1B Thr12, Phospho MOBKL1B

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Tested Applications

• Positive WB detected in: PC-12 cells, λ phosphatase treated PC-12 cells
• Positive IF/ICC detected in: λ phosphatase treated U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29027-1-AP targets Phospho-MOBKL1B (Thr12) in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: MOB1, Mps One Binder kinase activator-like 1B (yeast)
• Calculated Molecular Weight: 216 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC003398
• Gene Symbol: MOBKL1B
• Gene ID (NCBI): 55233
• RRID: AB_2923589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H8S9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. MOB1 protein is a key regulator of large tumor suppressor 1/2 (LATS1/2) kinases in the Hippo pathway. MOBKL1A and MOBKL1B are phosphorylated by MST1/MST2 kinases at Thr35 and Thr12, and MST1/MST2-catalyzed phosphorylation of MOBKL1A/MOBKL1B in intact cells is sufficient to substantially retard cell-cycle progression (PMID: 18328708).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	J Cell Physiol; m6 A-induced TRIB3 regulates Hippo pathway through interacting with LATS1 to promote the progression of lung adenocarcinoma; Authors - Jiamei Wu