Phospho-MOBKL1B (Thr12) Polyclonal antibody

Phospho-MOBKL1B (Thr12) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29027-1-AP

Synonyms

C2orf6, FLJ10788, FLJ11595, MATS1, MOB1, Mob1 alpha, Mob1 homolog 1B, Mob1A, MOB1B, Mob4B, MOBK1B, MOBKL1B, Phospho-MOB1B (Thr12), Phospho-MOBKL1B (Thr12), Protein Mob4B

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Validation Data Gallery

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  WB analysis using 29027-1-AP

  Phosphatase inhibitor treated and λ phosphatase treated PC-12 cells were subjected to SDS PAGE followed by western blot with 29027-1-AP (Phospho-MOBKL1B (Thr12) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: PC-12 cells, λ phosphatase treated PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29027-1-AP targets Phospho-MOBKL1B (Thr12) in WB, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: MOB1, Mps One Binder kinase activator-like 1B (yeast)
• Calculated Molecular Weight: 216 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC003398
• Gene Symbol: MOBKL1B
• Gene ID (NCBI): 55233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. MOB1 protein is a key regulator of large tumor suppressor 1/2 (LATS1/2) kinases in the Hippo pathway. MOBKL1A and MOBKL1B are phosphorylated by MST1/MST2 kinases at Thr35 and Thr12, and MST1/MST2-catalyzed phosphorylation of MOBKL1A/MOBKL1B in intact cells is sufficient to substantially retard cell-cycle progression (PMID: 18328708).