Phospho-MYL9 (Thr19/Ser20) Polyclonal antibody

Phospho-MYL9 (Thr19/Ser20) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


Human and More (1)





Cat no : 29504-1-AP


20 kDa myosin light chain, LC20, MLC 2C, MLC2, MRLC1, MYL9, Myosin RLC, MYRL2, Phospho-MYL9 (Thr18/Ser19), Phospho-MYL9 (Thr19/Ser20)

Tested Applications

Positive WB detected inCalyculin A treated HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29504-1-AP targets Phospho-MYL9 (Thr19/Ser20) in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, light chain 9, regulatory
Calculated Molecular Weight 20 kDa
Observed Molecular Weight19-20 kDa
GenBank Accession NumberBC002648
Gene Symbol MYL9
Gene ID (NCBI) 10398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).


Product Specific Protocols
WB protocol for Phospho-MYL9 (Thr19/Ser20) antibody 29504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Cell Dev Biol

Paracrine HB-EGF signaling reduce enhanced contractile and energetic state of activated decidual fibroblasts by rebalancing SRF-MRTF-TCF transcriptional axis

Authors - Junaid Afzal

Int J Mol Sci

Non-Muscle MLCK Contributes to Endothelial Cell Hyper-Proliferation through the ERK Pathway as a Mechanism for Vascular Remodeling in Pulmonary Hypertension

Authors - Mariam Anis