Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant antibody, PBS Only

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  WB analysis using 82717-1-RR (same clone as 82717-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 82717-1-PBS in a different storage buffer formulation.

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  WB analysis using 82717-1-RR (same clone as 82717-1-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 82717-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82717-1-RR (same clone as 82717-1-PBS)

  1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant antibody (82717-1-RR, Clone:1G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82717-1-PBS in a different storage buffer formulation.

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 82717-1-PBS

Clone No.1G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

MYL12A, MYL12B, P MYL12A, P MYL12B, p MYL9

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Product Information

82717-1-PBS targets Phospho-Myosin Light Chain 2 (Thr18/Ser19) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, light chain 9, regulatory
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 19-20 kDa
• GenBank Accession Number: BC002648
• Gene Symbol: MYL9
• Gene ID (NCBI): 10398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24844
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.