Phospho-NDRG1 (Thr346) Recombinant antibody, PBS Only

• 

  WB analysis of C2C12 using 85141-5-RR (same clone as 85141-5-PBS)

  Non-treated C2C12 cells and Insulin treated C2C12 cells were subjected to SDS PAGE followed by western blot with 85141-5-RR (Phospho-NDRG1 (Thr346) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85141-5-PBS in a different storage buffer formulation.

Phospho-NDRG1 (Thr346) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85141-5-PBS

Clone No.250220C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

NDRG1 (Thr346), p NDRG1, p NDRG1 (Thr346), Phospho NDRG1, Phospho NDRG1 (Thr346)

---

Product Information

85141-5-PBS targets Phospho-NDRG1 (Thr346) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: N-myc downstream regulated 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 46 kDa, 48 kDa
• GenBank Accession Number: BC006260
• Gene Symbol: NDRG1
• Gene ID (NCBI): 10397
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92597
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

N-Myc Downstream Regulated 1 (NDRG1) is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. The phosphorylation of NDRG1 (on Thr346) showed the greatest statistically significant increase in clear cell renal cell carcinoma (ccRCC) compared with noncancerous renal tissue. Further study showed the phosphorylation of NDRG1 (Thr346) in ccRCC cells was maintained by RICTOR and mTOR in complex 2. (PMID: 37298315)