Tested Applications
| Positive WB detected in | Calyculin A treated HEK-293T cells, Calyculin A treated PC-3 cells, HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28851-1-AP targets Phospho-Neurofibromin 2 (Ser518) in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | neurofibromin 2 (merlin) |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC007279 |
| Gene Symbol | NF2 |
| Gene ID (NCBI) | 4771 |
| RRID | AB_2935410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35240 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors. 28851-1-AP antibody is specific to phosphorylation of NF2 at serine 518. (PMID: 11782491, 14724586, 18361411, 18835652, 32115406)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Neurofibromin 2 (Ser518) antibody 28851-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-Neurofibromin 2 (Ser518) Polyclonal antibody (Cat# 28851-1-AP) has 2 total citations. These appear in J Cell Mol Med and Microorganisms. The cited research spans bladder cancer progression via ferroptosis inhibition and phosphoproteomics of baicalin-mediated cell cycle arrest in brain microvascular endothelial cells. Applications used include Western blot and IHC, with reactivity in human, mouse, and rat.
| Species | Application | Title |
|---|---|---|
J Cell Mol Med DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis | ||
Microorganisms Phosphoproteome Reveals the Role of Baicalin in Alleviating rPVL-Induced Cell Cycle Arrest in BMECs |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used it for Western Blot on Jurkat cells at a 1:1000 dilution and reported that it works well for WB. Overall, the feedback is positive, confirming reliable performance in Western Blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Titouan (Verified Customer) (11-12-2025) | Works for WB
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