Phospho-eNOS (Thr495) Polyclonal antibody

Phospho-eNOS (Thr495) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 28939-1-AP

Synonyms

NOS3, eNOS, EC-NOS, ECNOS, EC:1.14.13.39

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Validation Data Gallery

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  WB analysis using 28939-1-AP

  Non-treated, VEGF (HZ-1038), EGF and Cobalt Chloride treated HUVEC cells were subjected to SDS PAGE followed by western blot with 28939-1-AP (Phospho-NOS3 (Thr495) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: VEGF treated HUVEC cells, EGF treated HUVEC cells, Cobalt Chloride treated HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

28939-1-AP targets Phospho-eNOS (Thr495) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nitric oxide synthase 3 (endothelial cell)
• Calculated Molecular Weight: 133 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: BC063294
• Gene Symbol: eNOS
• Gene ID (NCBI): 4846
• RRID: AB_2881234
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine. The sequence homology of eNOS protein in human and mouse is above 90%, and phosphorylation of threonine at 495 site in human corresponds to 494 site in mouse.

Publications

Species	Application	Title
human	WB	Hypertension; Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia; Authors - Lunbo Tan
rat	WB	J Ethnopharmacol; Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway; Authors - Chuankui Fu
rat	WB	Drug Des Devel Ther; Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157.; Authors - Hailu Wu
human	WB	J Oncol; Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway.; Authors - Sha Wang
human	WB	Cytotherapy; Porous gelatin microspheres implanted with adipose mesenchymal stromal cells promote angiogenesis via protein kinase B/endothelial nitric oxide synthase signaling pathway in bladder reconstruction; Authors - Jun Zhao