Tested Applications
| Positive WB detected in | VEGF treated HUVEC cells, EGF treated HUVEC cells, Cobalt Chloride treated HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28939-1-AP targets Phospho-eNOS (Thr495) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | nitric oxide synthase 3 (endothelial cell) |
| Calculated Molecular Weight | 133 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC063294 |
| Gene Symbol | eNOS |
| Gene ID (NCBI) | 4846 |
| RRID | AB_2881234 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29474 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine. The sequence homology of eNOS protein in human and mouse is above 90%, and phosphorylation of threonine at 495 site in human corresponds to 494 site in mouse.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-eNOS (Thr495) antibody 28939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-eNOS (Thr495) Polyclonal antibody (Cat# 28939-1-AP) has 16 citations across journals including J Clin Invest, Hypertension, J Adv Res, Commun Biol, J Cell Mol Med, Cytotherapy, J Sex Med, and others. Research fields span angiogenesis, wound healing, hypertension, erectile dysfunction, liver cirrhosis, preeclampsia, gastric injury, myocardial infarction, bladder reconstruction, pulmonary arterial hypertension, uveal melanoma, and endothelial cell senescence.
| Species | Application | Title |
|---|---|---|
J Adv Res OTUD1 delays wound healing by regulating endothelial function and angiogenesis in diabetic mice | ||
J Clin Invest A smooth muscle cell lncRNA controls angiogenesis in chronic limb-threatening ischemia through miR-143-3p/HHIP signaling. | ||
Hypertension Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia | ||
Int J Biol Macromol A bioactive protein from Dioscorea polystachya residue alleviates hydrocortisone-induced erectile dysfunction in rats via TXNIP/NLRP3 pathway inhibition. | ||
J Ethnopharmacol Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway | ||
Bioresour Bioprocess Dendrobium officinale-derived nanovesicles: a natural therapy for comprehensive regulation of angiogenesis, inflammation, and tissue repair to enhance skin wound healing |
