Phospho-P53 (Ser15) Monoclonal antibody

Phospho-P53 (Ser15) Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1H6G1

Cat no : 67826-1-Ig

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser15), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

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Validation Data Gallery

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  WB analysis using 67826-1-Ig

  Non-treated and Calyculin A treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

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  WB analysis using 67826-1-Ig

  Non-treated and Etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

• 

  WB analysis using 67826-1-Ig

  Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

• 

  WB analysis using 67826-1-Ig

  Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 67826-1-Ig (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH and P53 antibody subsequently.

• 

  FC experiment of HT-29 using 67826-1-Ig

  1X10^6 HT-29 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (67826-1-Ig, Clone:1H6G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive WB detected in: Calyculin A treated HT-29 cells, HT-29 cells, HEK-293T cells, HEK-293T cells, Etoposide treated HT-29 cells
• Positive FC detected in: UV treated HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

67826-1-Ig targets Phospho-P53 (Ser15) in WB, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor protein p53
• Calculated molecular weight: 44 kDa
• Observed molecular weight: 53 kDa
• GenBank accession number: BC003596
• Gene symbol: TP53
• Gene ID (NCBI): 7157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Publications

Species	Application	Title
human	WB	Front Pharmacol; A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression.; Authors - Ting Wang
human	WB	Toxicol Lett; The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs]; Authors - Xiaoran Guan