Phospho-P53 (Ser15) Monoclonal antibody
Phospho-P53 (Ser15) Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1H6G1
Cat no : 67826-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Calyculin A treated HT-29 cells, HT-29 cells, HEK-293T cells, HEK-293T cells, Etoposide treated HT-29 cells |
Positive FC detected in | UV treated HT-29 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
67826-1-Ig targets Phospho-P53 (Ser15) in WB, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | tumor protein p53 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 53 kDa |
GenBank accession number | BC003596 |
Gene symbol | TP53 |
Gene ID (NCBI) | 7157 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-P53 (Ser15) antibody 67826-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Pharmacol A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression. | ||
Toxicol Lett The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs] |