Phospho-P53 (Ser46) Polyclonal antibody

• Home
• Products
• Primary Antibodies
• Phospho-P53 (Ser46) Polyclonal antibody (28960-1-AP)

Phospho-P53 (Ser46) Polyclonal Antibody for WB, ELISA

Cat No. 28960-1-AP Copied!

Publications (12)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser46), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

• 

  WB analysis using 28960-1-AP

  Non-treated HT-29, phosphatase inhibitor treated and λ phosphatase treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28960-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 28960-1-AP

  Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28959-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for Phospho-P53 (Ser46) in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HT-29 cells, λ phosphatase treated HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28960-1-AP targets Phospho-P53 (Ser46) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• RRID: AB_2918223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04637
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site .

Publications

What published studies show

The anti-p53 antibody (Cat# 28960-1-AP) has 12 citations spanning journals including Nature Communications, Journal for ImmunoTherapy of Cancer, Phytomedicine, Cell Death & Discovery, Apoptosis, Chemico-Biological Interactions, Toxicology, Microbial Pathogenesis, Cell Biology International, Experimental Cell Research, Molecular Cell Probes, and Tissue and Cell. Research fields cover acute kidney injury, gastric cancer, glioblastoma, intestinal stem cell homeostasis, apoptosis mechanisms, breast cancer, ovarian cancer, multiple myeloma, and viral pathogenesis.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312.; Authors - Ying Fu
		J Immunother Cancer; Targeting ATF4 overcomes dual resistance to chemo-immunotherapy in gastric cancer by disrupting the DNA damage response.; Authors - Huili Ye
human	WB	Phytomedicine; Rosmarinic acid attenuates glioblastoma cells and spheroids' growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis; Authors - Aslıhan Şengelen
mouse	WB	Cell Death Discov; p21 Regulates Wnt-Notch balance via DREAM/MMB/Rb-E2F1 and maintains intestinal stem cell homeostasis; Authors - Liangxia Jiang
human	WB	Apoptosis; High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines.; Authors - Chieh-Hsin Chen
human	WB	Chem Biol Interact; 5-Ethynyl-2'-deoxyuridine (EdU) regulates apoptosis, autophagy, and cell cycle dynamics in 2D and 3D U87-MG glioma cells.; Authors - Ceren Gencel Güler